microcon ym-100 centrifugal filter columns (Millipore)
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microcon ym-100 centrifugal filter columns
Microcon Ym 100 Centrifugal Filter Columns, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microcon+ym-100+column/pvdf+membrane/us11820994-1511-6-10
Average 90 stars, based on 1 article reviews
Microcon Ym 100 Centrifugal Filter Columns, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microcon+ym-100+column/pvdf+membrane/us11820994-1511-6-10
Average 90 stars, based on 1 article reviews
microcon ym-100 centrifugal filter columns - by Bioz Stars,
2026-09
90/100 stars
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Amplification:Article Title: Insight into the sialome of the castor bean tick, Ixodes ricinus Article Snippet: Each pooled cDNA was washed with ultrapure water and concentrated to 4–7 μl using Article Title: Flanking-sequence exponential anchored-polymerase chain reaction amplification: a sensitive and highly specific method for detecting retroviral integrant-host-junction sequences Article Snippet: Thermocycle parameters for linear PCR were: 95°C for 5 min (activate Taq) then 50 cycles of 95°C for 1 min, 60°C for 40 s and 72°C for 55 s. Next, small linear PCR fragments and unused primers were removed with a Article Title: Identification, High-Density Mapping, and Characterization of New Major Powdery Mildew Resistance Loci From the Emmer Wheat Landrace GZ1 Article Snippet: Before DNA sequencing, 100,000 flow-sorted chromosomes were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50°C, and DNA was purified using a Article Title: Metagenomes of the Picoalga Bathycoccus from the Chile Coastal Upwelling Article Snippet: The amplified products were then purified and concentrated using a Article Title: Method for detecting a target nucleic acid comprising two portions using probes having a first portion complementary to the first portion of the target nucleic acid and a second portion substantially complementary to the second portion of the target nucleic acid Article Snippet: Bring the sample to 100 ml by adding 28.75 ml 1×TE Buffer. c. Purification and Concentration of cDNA Purify the 100 μl of cDNA using a Article Title: The chromatin determinants and Ph1 gene effect at wheat sites with contrasting recombination frequency Article Snippet: The flow-sorted nuclei were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50 °C, and DNA was purified using a Article Title: FISHIS: Fluorescence In Situ Hybridization in Suspension and Chromosome Flow Sorting Made Easy Article Snippet: A total of 30,000–40,000 flow-sorted chromosomes were treated with 1 μl of proteinase K solution (20 mg/ml Qiagen, Germantown, MD, USA), in 30 mM Tris-HCl pH 8 at 50°C for 18 h, loaded onto a Article Title: The chromatin determinants and Ph1 gene effect at wheat sites with contrasting recombination frequency. Article Snippet: The flow-sorted nuclei were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50 C, and DNA was purified using a Purification:Article Title: Insight into the sialome of the castor bean tick, Ixodes ricinus Article Snippet: Each pooled cDNA was washed with ultrapure water and concentrated to 4–7 μl using Article Title: Flanking-sequence exponential anchored-polymerase chain reaction amplification: a sensitive and highly specific method for detecting retroviral integrant-host-junction sequences Article Snippet: Thermocycle parameters for linear PCR were: 95°C for 5 min (activate Taq) then 50 cycles of 95°C for 1 min, 60°C for 40 s and 72°C for 55 s. Next, small linear PCR fragments and unused primers were removed with a Article Title: Identification, High-Density Mapping, and Characterization of New Major Powdery Mildew Resistance Loci From the Emmer Wheat Landrace GZ1 Article Snippet: Before DNA sequencing, 100,000 flow-sorted chromosomes were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50°C, and DNA was purified using a Article Title: Metagenomes of the Picoalga Bathycoccus from the Chile Coastal Upwelling Article Snippet: The amplified products were then purified and concentrated using a Article Title: Method for detecting a target nucleic acid comprising two portions using probes having a first portion complementary to the first portion of the target nucleic acid and a second portion substantially complementary to the second portion of the target nucleic acid Article Snippet: Bring the sample to 100 ml by adding 28.75 ml 1×TE Buffer. c. Purification and Concentration of cDNA Purify the 100 μl of cDNA using a Article Title: The chromatin determinants and Ph1 gene effect at wheat sites with contrasting recombination frequency Article Snippet: The flow-sorted nuclei were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50 °C, and DNA was purified using a Article Title: FISHIS: Fluorescence In Situ Hybridization in Suspension and Chromosome Flow Sorting Made Easy Article Snippet: A total of 30,000–40,000 flow-sorted chromosomes were treated with 1 μl of proteinase K solution (20 mg/ml Qiagen, Germantown, MD, USA), in 30 mM Tris-HCl pH 8 at 50°C for 18 h, loaded onto a Article Title: The chromatin determinants and Ph1 gene effect at wheat sites with contrasting recombination frequency. Article Snippet: The flow-sorted nuclei were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50 C, and DNA was purified using a DNA Sequencing:Article Title: Insight into the sialome of the castor bean tick, Ixodes ricinus Article Snippet: Each pooled cDNA was washed with ultrapure water and concentrated to 4–7 μl using Article Title: Flanking-sequence exponential anchored-polymerase chain reaction amplification: a sensitive and highly specific method for detecting retroviral integrant-host-junction sequences Article Snippet: Thermocycle parameters for linear PCR were: 95°C for 5 min (activate Taq) then 50 cycles of 95°C for 1 min, 60°C for 40 s and 72°C for 55 s. Next, small linear PCR fragments and unused primers were removed with a Article Title: Identification, High-Density Mapping, and Characterization of New Major Powdery Mildew Resistance Loci From the Emmer Wheat Landrace GZ1 Article Snippet: Before DNA sequencing, 100,000 flow-sorted chromosomes were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50°C, and DNA was purified using a Article Title: Metagenomes of the Picoalga Bathycoccus from the Chile Coastal Upwelling Article Snippet: The amplified products were then purified and concentrated using a Article Title: Method for detecting a target nucleic acid comprising two portions using probes having a first portion complementary to the first portion of the target nucleic acid and a second portion substantially complementary to the second portion of the target nucleic acid Article Snippet: Bring the sample to 100 ml by adding 28.75 ml 1×TE Buffer. c. Purification and Concentration of cDNA Purify the 100 μl of cDNA using a Article Title: The chromatin determinants and Ph1 gene effect at wheat sites with contrasting recombination frequency Article Snippet: The flow-sorted nuclei were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50 °C, and DNA was purified using a Article Title: FISHIS: Fluorescence In Situ Hybridization in Suspension and Chromosome Flow Sorting Made Easy Article Snippet: A total of 30,000–40,000 flow-sorted chromosomes were treated with 1 μl of proteinase K solution (20 mg/ml Qiagen, Germantown, MD, USA), in 30 mM Tris-HCl pH 8 at 50°C for 18 h, loaded onto a Article Title: The chromatin determinants and Ph1 gene effect at wheat sites with contrasting recombination frequency. Article Snippet: The flow-sorted nuclei were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50 C, and DNA was purified using a Concentration Assay:Article Title: Insight into the sialome of the castor bean tick, Ixodes ricinus Article Snippet: Each pooled cDNA was washed with ultrapure water and concentrated to 4–7 μl using Article Title: Flanking-sequence exponential anchored-polymerase chain reaction amplification: a sensitive and highly specific method for detecting retroviral integrant-host-junction sequences Article Snippet: Thermocycle parameters for linear PCR were: 95°C for 5 min (activate Taq) then 50 cycles of 95°C for 1 min, 60°C for 40 s and 72°C for 55 s. Next, small linear PCR fragments and unused primers were removed with a Article Title: Identification, High-Density Mapping, and Characterization of New Major Powdery Mildew Resistance Loci From the Emmer Wheat Landrace GZ1 Article Snippet: Before DNA sequencing, 100,000 flow-sorted chromosomes were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50°C, and DNA was purified using a Article Title: Metagenomes of the Picoalga Bathycoccus from the Chile Coastal Upwelling Article Snippet: The amplified products were then purified and concentrated using a Article Title: Method for detecting a target nucleic acid comprising two portions using probes having a first portion complementary to the first portion of the target nucleic acid and a second portion substantially complementary to the second portion of the target nucleic acid Article Snippet: Bring the sample to 100 ml by adding 28.75 ml 1×TE Buffer. c. Purification and Concentration of cDNA Purify the 100 μl of cDNA using a Article Title: The chromatin determinants and Ph1 gene effect at wheat sites with contrasting recombination frequency Article Snippet: The flow-sorted nuclei were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50 °C, and DNA was purified using a Article Title: FISHIS: Fluorescence In Situ Hybridization in Suspension and Chromosome Flow Sorting Made Easy Article Snippet: A total of 30,000–40,000 flow-sorted chromosomes were treated with 1 μl of proteinase K solution (20 mg/ml Qiagen, Germantown, MD, USA), in 30 mM Tris-HCl pH 8 at 50°C for 18 h, loaded onto a Article Title: The chromatin determinants and Ph1 gene effect at wheat sites with contrasting recombination frequency. Article Snippet: The flow-sorted nuclei were treated with 60 ng/ml proteinase K (Roche, Basel, Switzerland) for 40 h at 50 C, and DNA was purified using a |